High throughput fluorescent CE-SSCP SNP genotyping

Mol Psychiatry. 1999 Jul;4(4):339-43. doi: 10.1038/sj.mp.4000564.

Abstract

Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and low cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Automation / methods
  • Chromosome Mapping / methods*
  • DNA Primers
  • Electrophoresis, Capillary / methods
  • Fluorescent Dyes
  • Genotype
  • Humans
  • Point Mutation
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic*
  • Polymorphism, Single-Stranded Conformational*
  • Proteins / genetics

Substances

  • DNA Primers
  • Fluorescent Dyes
  • Proteins