Tyrosine phosphorylation in type-1 diabetes by immunogold detection: an in vitro human aortic endothelial cell (HAEC) study in the presence of diabetic low density lipoproteins (LDL)

Eur J Histochem. 1999;43(3):199-204.

Abstract

An immunomorphometric study of tyrosine phosphorylation was performed by the immunogold technique on cultured human aortic endothelial cells (HAEC) with a view to demonstrating their impaired signal transduction status, induced in vitro by incubation with low-density lipoproteins from the plasma of Type-1 diabetic patients. The results seem to sustain the hypothesis that extranuclear bioenergetic derangement induced by low-density lipoproteins from Type-1 diabetic patients may be associated with an up-regulation of the nuclear energetic machinery aimed at maintaining intracellular metabolic equilibrium. Our data demonstrate that phosphorylated tyrosine is a useful marker to monitor this metabolic condition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cytoplasm / metabolism
  • Diabetes Mellitus, Type 1 / metabolism*
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / ultrastructure
  • Humans
  • Immunohistochemistry
  • Lipid Peroxides / metabolism
  • Lipoproteins, LDL / metabolism*
  • Nuclear Proteins / metabolism
  • Phosphorylation
  • Tyrosine / metabolism*

Substances

  • Lipid Peroxides
  • Lipoproteins, LDL
  • Nuclear Proteins
  • Tyrosine