Objective: To obtaining cDNA clone of the coding region of human augmenter of liver regeneration(hALR) as a basis for further study.
Methods: Total RNA of human fetal liver was extracted as a template. First strand of cDNA was synthesised by reverse transcription with olig(dT)-primer and then amplified by PCR with the primers designed by ourself. The size of PCR product was about 380 bp. it was subcloned into pUC19 and was sequenced by auto-sequencing instrument. The sequence of hALR was analysed with PCGENE program.
Result: The cDNA of integrity coding region of hALR was obtained. Its size was 378 bp. The sequence homology of our hALR compared with rat ALR and human ALR recently reported were 86.5% and 99.2%, respectively.
Conclusion: It was also suggested that hALR takes part in growth regulation of fetal liver during human fetus late development.