A lipid's eye view of membrane protein crystallization in mesophases

Curr Opin Struct Biol. 2000 Aug;10(4):486-97. doi: 10.1016/s0959-440x(00)00119-6.

Abstract

Lipidic mesophases have been used to produce diffraction-quality crystals of several membrane proteins. The mechanism by which the method works is a mystery. The thrust is to continue to use it whilst deciphering the underlying mechanism and solving the second 'phase problem' in membrane protein crystallography. The method, which probably shares similarities with crystal growth in microgravity, is examined here from a lipid and a phase science perspective.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Crystallization
  • Crystallography, X-Ray / methods
  • Humans
  • Membrane Lipids / chemistry*
  • Membrane Proteins / chemistry*
  • Protein Conformation

Substances

  • Membrane Lipids
  • Membrane Proteins