The structure of pariacoto virus reveals a dodecahedral cage of duplex RNA

Nat Struct Biol. 2001 Jan;8(1):77-83. doi: 10.1038/83089.

Abstract

The 3.0 A resolution crystal structure of Pariacoto virus (PaV) reveals extensive interactions between portions of the viral RNA genome and the icosahedral capsid. Under the protein shell of the T = 3 quasi equivalent capsid lies a dodecahedral cage composed of RNA duplex that accounts for approximately 35% of the single-stranded RNA genome. The highly basic N-terminal regions (residues 7-54) of the subunits, forming pentamers (A subunits) are clearly visible in the density map and make numerous interactions with the RNA cage. The C-terminal segments (residues 394-401) of the A subunits lie in channels near the quasi three-fold axes. Electron cryo-microscopy and image reconstruction of PaV particles clearly show the dodecahedral RNA cage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Capsid / chemistry
  • Capsid / genetics
  • Capsid / metabolism
  • Capsid / ultrastructure
  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Genome, Viral
  • Insect Viruses / chemistry
  • Insect Viruses / genetics*
  • Insect Viruses / ultrastructure*
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Conformation
  • Protein Structure, Quaternary
  • Protein Structure, Secondary
  • Protein Subunits
  • RNA Viruses / chemistry
  • RNA Viruses / genetics*
  • RNA Viruses / ultrastructure*
  • RNA, Viral / chemistry
  • RNA, Viral / genetics
  • RNA, Viral / ultrastructure*
  • Sequence Alignment
  • Spodoptera / virology*

Substances

  • Protein Subunits
  • RNA, Viral

Associated data

  • PDB/1F8V