Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay

Clin Diagn Lab Immunol. 2001 Jan;8(1):199-202. doi: 10.1128/CDLI.8.1.199-202.2001.

Abstract

Serodiagnostic tests are widely available for tick-borne diseases. We evaluated a cell-free antigen of the human granulocytic ehrlichiosis agent. Immunofluorescence assay (IFA) with this antigen is as efficient as with the MRL kit and allows a one-step IFA with other cell-free antigens that is useful when testing sera from patients bitten by ticks.

MeSH terms

  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / immunology
  • Ehrlichia / immunology
  • Ehrlichiosis / blood
  • Ehrlichiosis / diagnosis*
  • Ehrlichiosis / immunology
  • Ehrlichiosis / microbiology
  • Fluorescent Antibody Technique
  • Granulocytes
  • HL-60 Cells
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunoglobulin M / blood
  • Immunoglobulin M / immunology
  • Predictive Value of Tests
  • Serologic Tests

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M