Quantitative (31)P spectroscopic imaging of human brain at 4 Tesla: assessment of gray and white matter differences of phosphocreatine and ATP

Magn Reson Med. 2001 Jan;45(1):46-52. doi: 10.1002/1522-2594(200101)45:1<46::aid-mrm1008>3.0.co;2-n.

Abstract

This report describes the implementation and application of a multicompartment analysis of (31)P spectroscopic imaging data to determine the tissue-specific heterogeneities in metabolite content in the human brain and surrounding tissue. Using this information and a multicompartment regression analysis the phosphocreatine and ATP content of "pure" cerebral gray and white matter, the cerebellum, and skeletal muscle was determined in a group of 10 healthy volunteers. The data were converted to mM units using previously reported values for the T(1)s of phosphocreatine and ATP at 4 T, the water content of human brain, and an external reference for absolute quantification. The phosphocreatine concentration in cerebral gray and white matter, the cerebellum, and skeletal muscle was 3.53 +/- 0.33, 3.33 +/- 0.37, 3.75 +/- 0.66, and 25.8 +/- 2.3 mM, respectively. The ATP concentration in cerebral gray and white matter, the cerebellum, and skeletal muscle was 2.19 +/- 0.33, 3.41 +/- 0.33, 1.75 +/- 0.58, and 8.5 +/- 1.9 mM, respectively. Magn Reson Med 45:46-52, 2001.

MeSH terms

  • Adenosine Triphosphate / analysis*
  • Adult
  • Body Water
  • Brain Chemistry*
  • Cerebellum / chemistry
  • Cerebral Cortex / chemistry
  • Female
  • Humans
  • Magnetic Resonance Spectroscopy*
  • Male
  • Middle Aged
  • Muscle, Skeletal / chemistry
  • Phosphocreatine / analysis*

Substances

  • Phosphocreatine
  • Adenosine Triphosphate