DNA sequencing with transposons

J Comput Biol. 2000;7(5):717-29. doi: 10.1089/106652701446161.

Abstract

The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA. An important part of this process is the location of the transposon insertions (known as mapping) and the selection of a sensible subset of transposons to use as priming sites for sequencing reactions. This paper presents a probabilistic method of scoring selected subsets of transposons and a graph-theoretic algorithm for selection of a subset of maximal score.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • DNA Transposable Elements / genetics*
  • Models, Statistical
  • Sequence Analysis, DNA / methods*
  • Sequence Analysis, DNA / statistics & numerical data

Substances

  • DNA Transposable Elements