Abstract
Class A scavenger receptors (SR-A) mediate microglial interaction with fibrillar beta-amyloid (fAbeta). We report here that neonatal microglia from SR-A knockout mice (SR-A-/-) adhere to surface-bound fAbeta, and produce reactive oxygen species (ROS) as efficiently as wildtype microglia; that both wildtype and SR-A-/- microglia express SR-BI; that antibodies against SR-BI do not affect adhesion or ROS production by wildtype microglia, but inhibit adhesion and ROS production of SR-A-/- microglia to immobilized fAbeta by approximately 40%. Adhesion to fAbeta-coated surfaces, and uptake of fAbeta by both wildtype and SR-A-/- microglia was almost completely inhibited by incubation with fucoidan. Thus SR-BI and SR-A mediate similar effector functions in neonatal microglia, which suggests that SR-BI plays as important a role as SR-A, and can maintain the wildtype phenotype in SR-A-/- microglia.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alzheimer Disease / immunology
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Alzheimer Disease / metabolism
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Amyloid beta-Peptides / immunology
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Amyloid beta-Peptides / metabolism*
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Animals
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Animals, Newborn
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Antineoplastic Agents / pharmacology
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Brain / cytology
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CD36 Antigens / genetics*
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CD36 Antigens / immunology*
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CD36 Antigens / metabolism
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Carbocyanines / metabolism
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Carbocyanines / pharmacology
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Cell Adhesion / drug effects
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Cell Adhesion / immunology
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Cells, Cultured
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Fluorescent Dyes / metabolism
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Fluorescent Dyes / pharmacology
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Gene Expression / immunology
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Liver / metabolism
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Membrane Proteins*
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Microglia / cytology
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Microglia / immunology*
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Microglia / metabolism
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Peptide Fragments / immunology
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Peptide Fragments / metabolism*
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Polysaccharides / pharmacology
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Reactive Oxygen Species / metabolism
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Receptors, Immunologic*
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Receptors, Lipoprotein*
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Receptors, Scavenger
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Scavenger Receptors, Class A
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Scavenger Receptors, Class B
Substances
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Amyloid beta-Peptides
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Antineoplastic Agents
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CD36 Antigens
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Carbocyanines
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Fluorescent Dyes
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Membrane Proteins
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Peptide Fragments
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Polysaccharides
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Reactive Oxygen Species
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Receptors, Immunologic
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Receptors, Lipoprotein
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Receptors, Scavenger
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Scarb1 protein, mouse
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Scavenger Receptors, Class A
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Scavenger Receptors, Class B
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amyloid beta-protein (1-42)
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cyanine dye 3
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fucoidan