Scavenger receptor class B type I (SR-BI) mediates adhesion of neonatal murine microglia to fibrillar beta-amyloid

J Neuroimmunol. 2001 Mar 1;114(1-2):142-50. doi: 10.1016/s0165-5728(01)00239-9.

Abstract

Class A scavenger receptors (SR-A) mediate microglial interaction with fibrillar beta-amyloid (fAbeta). We report here that neonatal microglia from SR-A knockout mice (SR-A-/-) adhere to surface-bound fAbeta, and produce reactive oxygen species (ROS) as efficiently as wildtype microglia; that both wildtype and SR-A-/- microglia express SR-BI; that antibodies against SR-BI do not affect adhesion or ROS production by wildtype microglia, but inhibit adhesion and ROS production of SR-A-/- microglia to immobilized fAbeta by approximately 40%. Adhesion to fAbeta-coated surfaces, and uptake of fAbeta by both wildtype and SR-A-/- microglia was almost completely inhibited by incubation with fucoidan. Thus SR-BI and SR-A mediate similar effector functions in neonatal microglia, which suggests that SR-BI plays as important a role as SR-A, and can maintain the wildtype phenotype in SR-A-/- microglia.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alzheimer Disease / immunology
  • Alzheimer Disease / metabolism
  • Amyloid beta-Peptides / immunology
  • Amyloid beta-Peptides / metabolism*
  • Animals
  • Animals, Newborn
  • Antineoplastic Agents / pharmacology
  • Brain / cytology
  • CD36 Antigens / genetics*
  • CD36 Antigens / immunology*
  • CD36 Antigens / metabolism
  • Carbocyanines / metabolism
  • Carbocyanines / pharmacology
  • Cell Adhesion / drug effects
  • Cell Adhesion / immunology
  • Cells, Cultured
  • Fluorescent Dyes / metabolism
  • Fluorescent Dyes / pharmacology
  • Gene Expression / immunology
  • Liver / metabolism
  • Membrane Proteins*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Microglia / cytology
  • Microglia / immunology*
  • Microglia / metabolism
  • Peptide Fragments / immunology
  • Peptide Fragments / metabolism*
  • Polysaccharides / pharmacology
  • Reactive Oxygen Species / metabolism
  • Receptors, Immunologic*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B

Substances

  • Amyloid beta-Peptides
  • Antineoplastic Agents
  • CD36 Antigens
  • Carbocyanines
  • Fluorescent Dyes
  • Membrane Proteins
  • Peptide Fragments
  • Polysaccharides
  • Reactive Oxygen Species
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • amyloid beta-protein (1-42)
  • cyanine dye 3
  • fucoidan