[Enhancement of apoptotic sensitivity induced by UV irradiation on the p53-transducted K562 cells]

Zhonghua Xue Ye Xue Za Zhi. 1998 Dec;19(12):631-3.
[Article in Chinese]

Abstract

Objective: To answer whether wild-type p53 can sensitize K562 cell apoptosis induced by UV irradiation.

Methods: K562 cells transducted with retrovirus encoding wild-type p53 were irradiated by ultraviolet for different time and then cultured for different time. Apoptosis was detected by TDT end labelling technique, DNA fragmentation and MTT assay.

Results: TDT end labelling and DNA fragmentation showed that apoptosis induced by 8 min UV-irradiation differed significantly between K562-neo and K562-p53 cells.

Conclusion: Wild-type p53 can enhance apoptosis induced by UV-irradiation on K562 cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis* / genetics
  • Apoptosis* / radiation effects
  • Genes, p53*
  • Humans
  • K562 Cells / pathology
  • Retroviridae / genetics
  • Transfection
  • Tumor Suppressor Protein p53 / pharmacology*
  • Ultraviolet Rays

Substances

  • Tumor Suppressor Protein p53