Soluble apyrases release adp during ATP hydrolysis

Biochem Biophys Res Commun. 2001 Mar 23;282(1):90-5. doi: 10.1006/bbrc.2001.4555.

Abstract

A soluble form of CD39 was expressed and purified from High-Five insect cells. The soluble CD39 is a monomer with a molecular weight of 54,000. The k(cat) and K(m) of the purified soluble CD39 were 4.6 s(-1) and 12 microM for ATP and 1.3 s(-1) and 7 microM for ADP as substrates, respectively. One nucleotide binding site was detected on the monomer only in the presence of Ca(2+). In contrast to the membrane bound CD39, soluble CD39 released ADP as an intermediate during ATP hydrolysis, as did the soluble potato apyrase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Diphosphate / metabolism*
  • Adenosine Triphosphatases*
  • Adenosine Triphosphate / metabolism*
  • Amino Acid Sequence
  • Animals
  • Antigens, CD / isolation & purification
  • Antigens, CD / metabolism
  • Apyrase / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Hydrolysis
  • Insecta
  • Molecular Sequence Data
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Solubility

Substances

  • Antigens, CD
  • Recombinant Proteins
  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • Adenosine Triphosphatases
  • Apyrase
  • CD39 antigen