Isolation and initial characterization of the mouse Dnmt3l gene

Cytogenet Cell Genet. 2001;92(1-2):122-6. doi: 10.1159/000056881.

Abstract

We have isolated and sequenced the mouse zinc finger gene, Dnmt3l (DNA cytosine-5-methyltransferase 3-like), on mouse chromosome 10, showing similarity to members of the DNMT3/Dnmt3 family. The Dnmt3l protein contains an ADD zinc finger, which Dnmt3l shares with other Dnmt3 family members and Atrx. RT-PCR analysis showed Dnmt3l expression in testis, thymus, ovary, and heart, as well as in 7-day, 15-day, and 17-day mouse embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • DNA (Cytosine-5-)-Methyltransferases / analysis
  • DNA (Cytosine-5-)-Methyltransferases / chemistry
  • DNA (Cytosine-5-)-Methyltransferases / genetics*
  • Embryo, Mammalian / enzymology
  • Exons / genetics
  • Expressed Sequence Tags
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Ovary / enzymology
  • Physical Chromosome Mapping
  • Polymorphism, Single Nucleotide / genetics
  • Protein Structure, Tertiary
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Testis / enzymology
  • Thymus Gland / enzymology
  • Zinc Fingers

Substances

  • RNA, Messenger
  • Dnmt3l protein, mouse
  • DNA (Cytosine-5-)-Methyltransferases

Associated data

  • GENBANK/AJ404467