G-quadruplex DNA binding by a series of carbocyanine dyes

Bioorg Med Chem Lett. 2001 Sep 17;11(18):2411-4. doi: 10.1016/s0960-894x(01)00490-5.

Abstract

We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4'-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4'-DNA. DTC was also the only dye found to inhibit human telomerase at 50 microM concentration.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Carbocyanines / chemistry
  • Carbocyanines / metabolism*
  • Carbocyanines / pharmacology*
  • Coloring Agents / chemistry
  • Coloring Agents / metabolism*
  • Coloring Agents / pharmacology*
  • DNA / chemistry
  • DNA / metabolism*
  • Drug Evaluation, Preclinical
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism*
  • Enzyme Inhibitors / pharmacology*
  • HeLa Cells
  • Humans
  • Nucleic Acid Conformation
  • Taq Polymerase / antagonists & inhibitors
  • Telomerase / antagonists & inhibitors

Substances

  • Carbocyanines
  • Coloring Agents
  • Enzyme Inhibitors
  • N,N'-diethylthiacarbocyanine iodide
  • DNA
  • Taq Polymerase
  • Telomerase