Objective: To observe the changes of oxidation and hepatic cells apoptosis in the wet-heat syndrome rat model, and the effect of "qingxiangsan" on inhibiting oxidation and hepatic cells apoptosis.
Methods: Wistar rats were divided into wet-heat syndrome group, qingxiangsan group and normal control group. Flow cytometry and electron microscopy were employed to detect hepatic cells apoptosis. SOD and MDA in the serum, LPO in the hepatic tissue were also estimated.
Results: Apoptotic morphogenetic changes, apoptotic rate increasing, activity of SOD decreasing, content of MDA and LPO increasing were obvious in the wet-heat syndrome group. In the "qingxiangsan" group, apoptotic rate decreasing, activity of SOD increasing, content of MDA and LPO decreasing were examined.
Conclusion: "qingxiangsan" possesses the function of inhibiting oxidation and apoptosis in the wet-heat syndrome rat model.