Objective: To understand the information of donor and recipient in the mixed hematopoietic chimerism after non-myeloablative allogeneic peripheral blood stem cell transplantation(NM-APBSCT).
Methods: DNA samples were extracted with phenol/chloroform method and were amplified by PCR technique in heparin-blood or heparin-bone-marrow. The PCR products were analyzed using polyacrylamide gel electrophoresis and silver staining means.
Results: The amplified fragment length polymorphism was found in the short tandem repeat loci of 10 healthy persons and 8 leukemia patients who were not treated with hematopoietic stem cells transplantation; the bands of silver staining originated from donors and recipients were found in 3 patients after NM-APBSCT, but the brightness in the bands of donor and recipient was different.
Conclusion: Polymorphism for mixed hematopoietic chimerism can be estimated timely, sensitively and exactly; and the results may be used to guide adoptive immunotherapy for patients after NM-APBSCT. The silver means were simple without contamination of isotopes and without using special equipment. The methods may benefit common hospitals to develop work in this respect.