[Effect of rhTFAR19 protein on cell cycle and apoptosis of MCF-7 induced by gamma-ray]

Zhonghua Zhong Liu Za Zhi. 2000 Mar;22(2):102-4.
[Article in Chinese]

Abstract

Objective: To investigate the effect of protein product of TFAR19, an apoptosis-related gene previously cloned from leukemia cell line TF-1 cultured in medium deprived of growth factor, on cell cycle and apoptosis of human breast cancer induced by gamma-ray.

Methods: Cell morphology, fluorescence microscopy, laser scanning confocal microscopy, single-cell gel electrophoresis (SCGE) and flow cytometry (FCM) were applied to study cell cycle and apoptosis of breast cancer cell line MCF-7.

Results: rhTFAR19 protein itself had no effect on cell cycle arrest and apoptosis of MCF-7 cell induced by gamma-ray. When MCF-7 cells were pretreated with 10 micrograms/ml rhTFAR19 and then irradiated with 12 Gy gamma-ray, cell apoptosis was observed. It was characterized morphologically by chromatin condensation, nucleoar disintegration, formation of apoptotic bodies, and typical "cornet" on SCGE.

Conclusion: TFAR19 protein has no apparent effect on MCF-7 cell cycle induced by gamma-ray. However, it synergizes with gamma-radiation in the induction and enhancement of apoptosis.

Publication types

  • English Abstract

MeSH terms

  • Apoptosis Regulatory Proteins
  • Apoptosis*
  • Breast Neoplasms
  • Cell Cycle / drug effects
  • Cell Cycle / radiation effects
  • Female
  • Flow Cytometry
  • Gamma Rays*
  • Humans
  • Neoplasm Proteins / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Apoptosis Regulatory Proteins
  • Neoplasm Proteins
  • PDCD5 protein, human