Abstract
Chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI, or NR2F1) is an orphan nuclear receptor that plays a major role in the development of the nervous system. We show here that three ETS response elements in the COUP-TFI promoter mediate its transcription. A reporter gene containing these ETS binding sites is activated by Ets-1, while the same reporter with point mutations on all three ETS response elements is not. We also show that Ets-1 binds to these response elements and that other ETS factors also transactivate the COUP-TFI promoter. In addition, COUP-TFI is coexpressed with some ETS factors in the mouse embryo. These results indicate that members of the ETS family can activate COUP-TFI gene expression.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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COUP Transcription Factor I
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics*
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Embryo, Mammalian / chemistry
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Gene Expression Regulation*
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HeLa Cells
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Humans
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / physiology*
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Proto-Oncogene Proteins c-ets
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Response Elements
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Transcription Factors / analysis
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Transcription Factors / genetics*
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Transcription Factors / physiology*
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Transcriptional Activation
Substances
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COUP Transcription Factor I
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DNA-Binding Proteins
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ETS1 protein, human
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Ets1 protein, mouse
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NR2F1 protein, human
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Nr2f1 protein, mouse
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Transcription Factors