Analysis of the role of RecQ helicases in RNAi in mammals

Biochem Biophys Res Commun. 2002 Mar 15;291(5):1119-22. doi: 10.1006/bbrc.2002.6578.

Abstract

The identity of mammalian genes involved in RNA interference (RNAi), the targeted sequence-specific mRNA degradation by double-stranded RNA (dsRNA), is poorly defined. Here we report the analysis of mice with null mutations of Wrn, Blm, and RecQ1 genes that are related to Mut-7 and Qde3, two genes essential for RNAi in Caenorhabditis elegans and quelling in Neurospora, respectively. Our results suggest that Wrn, Blm, and RecQ1 are not involved in sequence-specific mRNA degradation in mammals in response to dsRNA, suggesting potential differences in the mammalian RNAi pathway.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / physiology
  • Animals
  • Caenorhabditis elegans / genetics
  • DNA Helicases / chemistry
  • DNA Helicases / genetics*
  • DNA Helicases / physiology
  • Exodeoxyribonucleases
  • Fungal Proteins*
  • Mice
  • Neurospora / genetics
  • Phylogeny
  • RNA, Double-Stranded / metabolism*
  • RNA, Messenger / metabolism
  • RecQ Helicases
  • Werner Syndrome Helicase

Substances

  • Fungal Proteins
  • RNA, Double-Stranded
  • RNA, Messenger
  • Exodeoxyribonucleases
  • Adenosine Triphosphatases
  • Bloom syndrome protein
  • RECQL protein, human
  • DNA Helicases
  • RecQ Helicases
  • WRN protein, human
  • Werner Syndrome Helicase
  • QDE-3 protein, Neurospora crassa