The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation

J Cell Sci. 2002 Apr 15;115(Pt 8):1759-68. doi: 10.1242/jcs.115.8.1759.

Abstract

The NIMA kinases are one of several families of kinases that participate in driving the eukaryotic cell cycle. NIMA-related kinases have been implicated in G2/M progression, chromatin condensation and regulation of the centrosome cycle. Here we report the identification of a new member of this family, FA2, from Chlamydomonas reinhardtii. FA2 was originally discovered in a genetic screen for deflagellation-defective mutants. We have previously shown that FA2 is essential for basal-body/centriole-associated microtubule severing. We now report that the FA2 NIMA-related kinase also plays a role in cell cycle progression in Chlamydomonas. This is the first indication that members of the NIMA family might exert their effects through the regulation of microtubule severing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle / physiology*
  • Cell Cycle Proteins*
  • Cell Separation
  • Cell Size
  • Chlamydomonas / genetics*
  • Chlamydomonas / metabolism
  • Flagella / metabolism*
  • Flow Cytometry
  • Genes, Protozoan*
  • Humans
  • Microtubules / metabolism*
  • Molecular Sequence Data
  • Multigene Family
  • NIMA-Related Kinase 1
  • Protein Serine-Threonine Kinases / chemistry
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / metabolism*
  • Sequence Alignment

Substances

  • Cell Cycle Proteins
  • Protozoan Proteins
  • NEK1 protein, human
  • NIMA-Related Kinase 1
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/AF479588