Abstract
Interleukin-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that lacks a transmembrane domain. IL-18BP binds specifically to mature IL-18 and inhibits its activity. To study the immunomodulating role of IL-18BP in models of autoimmune diseases in rats, we cloned and characterized rat IL-18BP. Rat IL-18BP has 193 amino acid residues and is highly homologous to human and mouse IL-18BP. Recombinant rat IL-18BP binds to rat IL-18, reacts with antibodies to human or mouse IL-18BP, and inhibits IL-18-dependent interferon-gamma (IFN-gamma) production in vitro. Thus, rat IL-18BP can be employed to antagonize the proinflammatory responses induced by endogenous IL-18 in rat models of autoimmune diseases.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
COS Cells
-
Cloning, Molecular
-
Dose-Response Relationship, Drug
-
Escherichia coli / genetics
-
Gene Expression
-
Glycoproteins / chemistry*
-
Glycoproteins / genetics
-
Glycoproteins / metabolism
-
Glycoproteins / pharmacology
-
Humans
-
Intercellular Signaling Peptides and Proteins
-
Interferon-gamma / biosynthesis
-
Interferon-gamma / drug effects
-
Interleukin-12 / pharmacology
-
Interleukin-18 / metabolism
-
Molecular Sequence Data
-
Rats
-
Rats, Inbred Lew
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / isolation & purification
-
Recombinant Proteins / metabolism
-
Recombinant Proteins / pharmacology
-
Sequence Homology, Amino Acid
Substances
-
Glycoproteins
-
Intercellular Signaling Peptides and Proteins
-
Interleukin-18
-
Recombinant Proteins
-
interleukin-18 binding protein
-
Interleukin-12
-
Interferon-gamma