Isolation and characterization of Leishmania infantum cDNA encoding a protein homologous to eukaryotic elongation factor 1 gamma

Trans R Soc Trop Med Hyg. 2002 Apr:96 Suppl 1:S41-7. doi: 10.1016/s0035-9203(02)90050-7.

Abstract

This paper reports the isolation and characterization of a complementary deoxyribonucleic acid clone showing sequence homology with genes coding for the eukaryotic elongation factor 1 gamma (EF-1 gamma). The clone encodes an open reading frame of 404 amino acids corresponding to a deduced molecular mass of 46.2 kDa. Database searches revealed 30-64% sequence identity between the Leishmania infantum EF-1 gamma and several eukaryotic homologues. Southern blot analysis indicated that 2 genes tandemly organized were present in the L. infantum genome. The 3' untranslated regions of these 2 genes differed in size. Southern hybridization and pulsed field gel electrophoresis showed that EF-1 gamma genes are highly conserved among members of the Leishmania genus and must be clustered in a single chromosomal locus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Conserved Sequence
  • DNA, Complementary / isolation & purification*
  • DNA, Protozoan / isolation & purification*
  • Electrophoresis, Gel, Pulsed-Field
  • Genome, Protozoan
  • Humans
  • Leishmania infantum / genetics*
  • Molecular Sequence Data
  • Peptide Elongation Factor 1 / genetics*
  • Protozoan Proteins / genetics*
  • Species Specificity

Substances

  • DNA, Complementary
  • DNA, Protozoan
  • Peptide Elongation Factor 1
  • Protozoan Proteins