Cytokines regulate c-Met expression in cultured astrocytes

Brain Res. 2003 Feb 7;962(1-2):105-10. doi: 10.1016/s0006-8993(02)03975-6.

Abstract

We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. Reverse transcription-polymerase chain reaction revealed that FGFs and HGF enhanced c-met expression. These findings suggest that c-Met expressed in astrocytes may have important roles during the nervous system regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Astrocytes / physiology*
  • Cells, Cultured
  • Cytokines / pharmacology*
  • Epidermal Growth Factor / pharmacology
  • Fibroblast Growth Factor 1 / pharmacology
  • Fibroblast Growth Factor 2 / pharmacology
  • Hepatocyte Growth Factor / pharmacology
  • Interleukin-1 / pharmacology
  • Nerve Regeneration
  • Proto-Oncogene Proteins c-met / genetics*
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Cytokines
  • Interleukin-1
  • Tumor Necrosis Factor-alpha
  • Fibroblast Growth Factor 2
  • Fibroblast Growth Factor 1
  • Epidermal Growth Factor
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met