Molecular patchwork: Chromosomal recombination between two Helicobacter pylori strains during natural colonization

Leonard C Smeets; Nicolaas L A Arents; Anton A van Zwet; Christina M J E Vandenbroucke-Grauls; Theo Verboom; Wilbert Bitter; Johannes G Kusters

doi:10.1128/IAI.71.5.2907-2910.2003

Molecular patchwork: Chromosomal recombination between two Helicobacter pylori strains during natural colonization

Infect Immun. 2003 May;71(5):2907-10. doi: 10.1128/IAI.71.5.2907-2910.2003.

Authors

Leonard C Smeets¹, Nicolaas L A Arents, Anton A van Zwet, Christina M J E Vandenbroucke-Grauls, Theo Verboom, Wilbert Bitter, Johannes G Kusters

Affiliation

¹ Department of Medical Microbiology and Infection Control, Vrije Universiteit Medical Center, Amsterdam, The Netherlands. lc.smeets@vumc.nl

Abstract

Genetic analysis of two Helicobacter pylori strains isolated from a single gastric biopsy showed evidence of extensive horizontal gene transfer. Several large recombinations were identified in the rdxA gene, which is involved in metronidazole resistance.

MeSH terms

Alleles
Base Sequence
Chromosomes, Bacterial*
Drug Resistance, Bacterial
Gastric Mucosa / microbiology*
Helicobacter pylori / drug effects
Helicobacter pylori / genetics*
Metronidazole / pharmacology
Molecular Sequence Data
Nitroreductases / genetics
Recombination, Genetic*

Substances

Metronidazole
aromatic NADH-dependent nitroreductase
Nitroreductases