Anticlastogenic effects of 13-cis-retinoic acid in vitro

Eur J Cancer. 1992;29A(1):137-40. doi: 10.1016/0959-8049(93)90592-4.

Abstract

The anticlastogenic effects of 13-cis-retinoic acid were studied in four human lymphoblastoid cell lines and in primary lymphocyte cultures derived from the peripheral blood of 11 study subjects. Cells were pre-incubated with 13-cis-retinoic acid in the concentration range of 10(-8)-10(-5) mol/l for 24 h and the numbers of chromatid breaks per cell induced by bleomycin were determined. The presence of 13-cis-retinoic acid decreased the number of breaks per cell by 13.0 to 59.5% in lymphoblastoid cell lines and by 0 to 57.4% in primary lymphocyte cultures (in the concentration ranges of 10(-8)-10(-6) mol/l and of 10(-8)-10(-5) mol/l, respectively). Regression analysis showed that there was a statistically significant correlation between the presence of 13-cis-retinoic acid and protection against bleomycin-induced clastogenicity. These data give additional information to the knowledge of possible chemopreventive mechanisms of action of 13-cis-retinoic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimutagenic Agents / pharmacology*
  • Bleomycin / antagonists & inhibitors
  • Chromosomes / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Isotretinoin / pharmacology*
  • Lymphocytes / drug effects
  • Male
  • Tumor Cells, Cultured / drug effects

Substances

  • Antimutagenic Agents
  • Bleomycin
  • Isotretinoin