Citrus psorosis virus RNA 1 is of negative polarity and potentially encodes in its complementary strand a 24K protein of unknown function and 280K putative RNA dependent RNA polymerase

Virus Res. 2003 Oct;96(1-2):49-61. doi: 10.1016/s0168-1702(03)00172-2.

Abstract

Citrus psorosis virus (CPsV), the type member of genus Ophiovirus, has three genomic RNAs. Complete sequencing of CPsV RNA 1 revealed a size of 8184 nucleotides and Northern blot hybridization with chain specific probes showed that its non-coding strand is preferentially encapsidated. The complementary strand of RNA 1 contains two open reading frames (ORFs) separated by a 109-nt intergenic region, one located near the 5'-end potentially encoding a 24K protein of unknown function, and another of 280K containing the core polymerase motifs characteristic of viral RNA-dependent RNA polymerases (RdRp). Comparison of the core RdRp motifs of negative-stranded RNA viruses, supports grouping CPsV, Ranunculus white mottle virus (RWMV) and Mirafiori lettuce virus (MiLV) within the same genus (Ophiovirus), constituting a monophyletic group separated from all other negative-stranded RNA viruses. Furthermore, RNAs 1 of MiLV, CPsV and RWMV are similar in size and those of MiLV and CPsV also in genomic organization and sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Citrus / virology*
  • Genes, Viral*
  • Genome, Viral
  • Molecular Sequence Data
  • Molecular Weight
  • Open Reading Frames
  • Phylogeny
  • Plant Diseases / virology
  • Plant Viruses / chemistry
  • Plant Viruses / genetics
  • RNA Viruses / chemistry
  • RNA Viruses / classification
  • RNA Viruses / genetics*
  • RNA-Dependent RNA Polymerase / genetics*

Substances

  • RNA-Dependent RNA Polymerase

Associated data

  • GENBANK/AY224663