Method for the quantitative assay of fatty acid-bile acid conjugates by tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Sep 25;795(1):35-40. doi: 10.1016/s1570-0232(03)00494-x.

Abstract

Fatty acid-bile acid conjugates and especially arachidyl amido cholic acid are synthetic molecules that were shown to prevent cholesterol gallstone formation in mice and hamsters as well as to dissolve pre-existing gallstones in mice. To measure these novel compounds we developed a liquid chromatography electrospray tandem mass spectrometry method based on the analysis of 100 microL of plasma with stearyl amido cholic acid (stamchol, 1.5 microM/L) added as internal standard. Repeatable calibrations between 0 and 50 microM/L exhibited consistent linearity and reproducibility. Inter- and intraassay C.V.s were 5.3-11.4% and 2.6-6.4%, respectively, at targeted concentrations of 0.1, 2.3 and 50 microM/L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bile Acids and Salts / analysis
  • Bile Acids and Salts / chemistry*
  • Calibration
  • Cricetinae
  • Fatty Acids / analysis
  • Fatty Acids / chemistry*
  • Mass Spectrometry / methods*
  • Mice
  • Reference Standards
  • Reproducibility of Results

Substances

  • Bile Acids and Salts
  • Fatty Acids