PCR-assisted localization of the human SRPR gene

Hum Genet. 1992 Mar;88(5):583-5. doi: 10.1007/BF00219349.

Abstract

A polymerase chain reaction (PCR)-based assay has been designed that detects the presence of the human signal recognition particle receptor (SRPR) gene in inter-species somatic cell hybrids. By using hybrids containing various fragments of chromosome 11q, SRPR has been mapped to a chromosomal region flanked by the 11q23 and 11q24 breakpoints associated with the constitutional and neuroepithelioma (11;22) translocations, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 22
  • DNA / genetics
  • DNA / isolation & purification
  • Electrophoresis, Agar Gel
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Neuroectodermal Tumors, Primitive, Peripheral / genetics
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • Ribonucleoproteins / genetics*
  • Signal Recognition Particle
  • Translocation, Genetic

Substances

  • Oligodeoxyribonucleotides
  • Ribonucleoproteins
  • Signal Recognition Particle
  • DNA