Abstract
The possible effect of oxidized HDL was investigated on lymphoblastoid cells, in comparison to the cytotoxic effect of oxidized LDL. Oxidation of HDL was promoted by UV-C irradiation, or by copper ion (5 microM) or the combination of the two treatments. HDL extensively treated by UV-C for 20 h did not exhibit any cytotoxic effect on cultured lymphoblastoid cells even at a concentration of 500 micrograms apolipoprotein A-I/ml. In contrast to UV-treated (2 h) LDL, which were highly cytotoxic (already at a concentration of 100 micrograms apolipoprotein B/ml), HDL treated by copper or copper + UV were oxidized, as shown by TBARS formation and PUFA content decrease, but were slightly cytotoxic.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Arteriosclerosis / etiology
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Cell Line / drug effects
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Copper
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Copper Sulfate
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Fatty Acids, Unsaturated / analysis
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Humans
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L-Lactate Dehydrogenase / analysis
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Lipoproteins, HDL / chemistry
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Lipoproteins, HDL / toxicity*
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Lipoproteins, LDL / chemistry
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Lipoproteins, LDL / toxicity*
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Oxidation-Reduction
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Tetrazolium Salts
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Thiazoles
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Thiobarbituric Acid Reactive Substances / analysis
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Trypan Blue
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Ultraviolet Rays
Substances
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Fatty Acids, Unsaturated
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Lipoproteins, HDL
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Lipoproteins, LDL
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Tetrazolium Salts
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Thiazoles
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Thiobarbituric Acid Reactive Substances
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Copper
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L-Lactate Dehydrogenase
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thiazolyl blue
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Trypan Blue
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Copper Sulfate