A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces

Peter B O'Connor; Bogdan A Budnik; Vera B Ivleva; Parminder Kaur; Susanne C Moyer; Jason L Pittman; Catherine E Costello

doi:10.1016/j.jasms.2003.10.005

A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces

J Am Soc Mass Spectrom. 2004 Jan;15(1):128-32. doi: 10.1016/j.jasms.2003.10.005.

Authors

Peter B O'Connor¹, Bogdan A Budnik, Vera B Ivleva, Parminder Kaur, Susanne C Moyer, Jason L Pittman, Catherine E Costello

Affiliation

¹ Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA. poconnor@bu.edu

PMID: 14698563
DOI: 10.1016/j.jasms.2003.10.005

Abstract

A new design for a high pressure MALDI-FTMS instrument is described and initial data are shown. The instrument incorporates a large, 10 cm x 10 cm, sample translation stage to accommodate and position the MALDI target. The new instrument allows coupling to a wide variety of surface techniques such as gel electrophoresis or surface plasmon resonance. Coupling to thin layer chromatography is shown. Furthermore, a new nozzle design allows high pressure collisional cooling sufficient to stabilize gangliosides while minimizing the gas load on the system.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Chromatography, Thin Layer
Gangliosides / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation*
Spectroscopy, Fourier Transform Infrared / instrumentation*

Substances

Gangliosides

Grants and funding

N01-HV-28178/HV/NHLBI NIH HHS/United States