Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system

Lauren S Cowan; Lois Diem; Mary Catherine Brake; Jack T Crawford

doi:10.1128/JCM.42.1.474-477.2004

Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system

J Clin Microbiol. 2004 Jan;42(1):474-7. doi: 10.1128/JCM.42.1.474-477.2004.

Authors

Lauren S Cowan¹, Lois Diem, Mary Catherine Brake, Jack T Crawford

Affiliation

¹ Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. LCowan@cdc.gov

Abstract

Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Genotype
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid