Osmotic stress leads to decreased intracellular pH of Listeria monocytogenes as determined by fluorescence ratio-imaging microscopy

Weihuan Fang; Henrik Siegumfeldt; Birgitte Bjørn Budde; Mogens Jakobsen

doi:10.1128/AEM.70.5.3176-3179.2004

Osmotic stress leads to decreased intracellular pH of Listeria monocytogenes as determined by fluorescence ratio-imaging microscopy

Appl Environ Microbiol. 2004 May;70(5):3176-9. doi: 10.1128/AEM.70.5.3176-3179.2004.

Authors

Weihuan Fang¹, Henrik Siegumfeldt, Birgitte Bjørn Budde, Mogens Jakobsen

Affiliation

¹ College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, People's Republic of China.

Abstract

Intracellular pH (pH(i)) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pH(i), and recovery on solid medium was impaired compared to that in liquid medium. N,N'-dicyclohexylcarbodiimide abolished pH(i) recovery, and lowering a(w) with glycerol showed no effect on pH(i).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Culture Media
Heat-Shock Response*
Hydrogen-Ion Concentration
Listeria monocytogenes / physiology*
Microscopy, Fluorescence / methods
Osmotic Pressure
Sodium Chloride / pharmacology*
Sorbitol / pharmacology*

Substances

Culture Media
Sodium Chloride
Sorbitol