Evaluation of different combinations of gated trapping, RF-only mode and trap compensation for in-field MALDI Fourier transform mass spectrometry

J Am Soc Mass Spectrom. 2004 Jul;15(7):1109-15. doi: 10.1016/j.jasms.2004.05.006.

Abstract

MALDI, while providing advantages such as the ability to do in-depth and repeated exploration of the sample, challenges the existing performance capabilities of Fourier transform mass spectrometry (FTMS). The challenge arises because MALDI-produced ions have high mass-to-charge ratios and uncertain kinetic-energy distributions. We demonstrate that a combination of a gated trapping event, a RF-only mode pressure focusing event, and an electrically compensated trap provides a compelling advantage in meeting these challenges. Removal of any of the above combination elements significantly degrades the detection performance of substance P from 850 K resolving power at 34.9 kHz and of melittin from 278 K resolving power at 16.5 kHz when using a 3-Tesla magnet-based spectrometer.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • Mass Spectrometry / methods*
  • Melitten / analysis
  • Melitten / chemistry*
  • Radio Waves
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Spectroscopy, Fourier Transform Infrared / instrumentation
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Spin Trapping / instrumentation
  • Spin Trapping / methods
  • Substance P / analysis
  • Substance P / chemistry*

Substances

  • Melitten
  • Substance P