Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

World J Gastroenterol. 2004 Sep 1;10(17):2560-2. doi: 10.3748/wjg.v10.i17.2560.

Abstract

Aim: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.

Methods: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.

Results: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.

Conclusion: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Adhesins, Bacterial / immunology*
  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology
  • Bacterial Vaccines / genetics*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Gene Expression
  • Helicobacter Infections / prevention & control*
  • Helicobacter Infections / therapy
  • Mice
  • Mice, Inbred BALB C
  • Plasmids
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Restriction Mapping

Substances

  • Adhesins, Bacterial
  • Antigens, Bacterial
  • BabA protein, Helicobacter pylori
  • Bacterial Vaccines
  • Recombinant Proteins