Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes

Sivan Dahan; Stuart Knutton; Robert K Shaw; Valerie F Crepin; Gordon Dougan; Gad Frankel

doi:10.1128/IAI.72.9.5452-5459.2004

Transcriptome of enterohemorrhagic Escherichia coli O157 adhering to eukaryotic plasma membranes

Infect Immun. 2004 Sep;72(9):5452-9. doi: 10.1128/IAI.72.9.5452-5459.2004.

Authors

Sivan Dahan¹, Stuart Knutton, Robert K Shaw, Valerie F Crepin, Gordon Dougan, Gad Frankel

Affiliation

¹ Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, United Kingdom.

Abstract

Using a DNA microarray, we determined changes in enterohemorrhagic Escherichia coli O157:H7 gene expression during binding to plasma membranes. Analysis of the complete transcriptomes of the bound bacteria revealed increased levels of stress-associated mRNAs and decreased levels of mRNA encoding proteins involved in translation and type III secretion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Adhesion
Cell Membrane / microbiology*
Erythrocytes / microbiology
Escherichia coli Infections / microbiology
Escherichia coli O157 / genetics
Escherichia coli O157 / metabolism
Escherichia coli O157 / physiology*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Eukaryotic Cells / microbiology
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Genome, Bacterial
Heat-Shock Response
Humans
Oligonucleotide Array Sequence Analysis / methods*
Rabbits
Transcription, Genetic*

Substances

Escherichia coli Proteins