Abstract
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacteria / genetics
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Bacteria / isolation & purification
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Computational Biology
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Computer Simulation
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DNA, Concatenated / genetics*
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Deoxyribonucleases, Type II Site-Specific / metabolism*
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Genome, Human
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Genomic Library
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Genomics / methods*
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Humans
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Karyotyping / methods*
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Markov Chains
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Polymerase Chain Reaction
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Viruses / genetics
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Viruses / isolation & purification
Substances
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DNA, Concatenated
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Deoxyribonucleases, Type II Site-Specific