cDNA cloning of translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) from the intertidal harpacticoid copepod Tigriopus japonicus

DNA Seq. 2004 Apr;15(2):159-63. doi: 10.1080/1042517042000199960.

Abstract

We synthesized a cDNA library from the intertidal copepod Tigriopus japonicus, converted it to phagemids and sequenced expressed sequence tags (ESTs). Of these, Tigriopus translationally controlled tumor protein/histamine releasing factor (TCTP/HRF) was further characterized. The Tigriopus TCTP/HRF gene encoded 172 amino acid residues and showed high similarity to Drosophila but moderate similarity to other annelids (e.g. Brugia, Wuchereria and C. elegans). The Tigriopus TCTP/HRF gene appeared in the same clade as the annelids. Here, we describe the analysis of the Tigriopus TCTP/HRF gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biomarkers, Tumor / genetics*
  • Cloning, Molecular
  • Copepoda / genetics*
  • DNA, Complementary / analysis
  • Gene Expression Regulation*
  • Molecular Sequence Data
  • Protein Biosynthesis*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Tumor Protein, Translationally-Controlled 1

Substances

  • Biomarkers, Tumor
  • DNA, Complementary
  • Tumor Protein, Translationally-Controlled 1

Associated data

  • GENBANK/AY496449