Genomic structure of the gene for copper/zinc-superoxide dismutase in rice

FEBS Lett. 1992 Apr 20;301(2):185-9. doi: 10.1016/0014-5793(92)81244-g.

Abstract

We have isolated and determined the nucleotide sequence of the structural gene (sodB) coding for one of the copper/zinc-superoxide dismutase (Cu/Zn-SOD) isozymes from rice plants (Oryza sativa L.). The sodB gene is split into 8 exons spread about 2 kb of the chromosomal DNA, with the coding sequence beginning in the 2nd and ending in the 8th. Although none of sodB introns are inserted into similar positions in Cu/Zn-SOD genes from other sources, four of six introns in the protein-coding region are located at discriminating positions within structural domains of the protein. Genomic Southern analysis indicated that cytosolic Cu/Zn-SOD is coded for by 2-3 genes in the rice genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • DNA
  • Exons
  • Humans
  • Introns
  • Molecular Sequence Data
  • Oryza / enzymology*
  • Oryza / genetics
  • Sequence Alignment
  • Superoxide Dismutase / genetics*
  • Superoxide Dismutase / metabolism

Substances

  • DNA
  • Superoxide Dismutase