Transcription regulation by bacteriophage T4 AsiA

Protein Expr Purif. 2005 May;41(1):1-8. doi: 10.1016/j.pep.2004.09.019.

Abstract

Bacteriophage T4 AsiA, a strong inhibitor of bacterial RNA polymerase, was the first antisigma protein to be discovered. Recent advances that made it possible to purify large amounts of this highly toxic protein led to an increased understanding of AsiA function and structure. In this review, we discuss how the small 10-KDa AsiA protein plays a key role in T4 development through its ability to both inhibit and activate bacterial RNA polymerase transcription.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Bacteriophage T4 / genetics*
  • Bacteriophage T4 / metabolism*
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / virology
  • Models, Biological
  • Transcription, Genetic
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • AsiA protein, Enterobacteria phage T4
  • Viral Proteins
  • DNA-Directed RNA Polymerases