Administration of epinephrine does not increase learning of fear to tone in rats anesthetized with isoflurane or desflurane

Anesth Analg. 2005 May;100(5):1333-1337. doi: 10.1213/01.ANE.0000148619.77117.C7.

Abstract

Previous reports suggest that the administration of epinephrine increases learning during deep barbiturate-chloral hydrate anesthesia in rats but not during anesthesia with 0.4% isoflurane in rabbits. We revisited this issue, using fear conditioning to a tone in rats as our experimental model for learning and memory and isoflurane and desflurane as our anesthetics. Expressed as a fraction of the minimum alveolar anesthetic concentration (MAC) preventing movement in 50% of rats, the amnestic 50% effective dose (ED(50)) for fear to tone in control rats inhaling isoflurane and injected with saline intraperitoneally (i.p.) was 0.32 +/- 0.03 MAC (mean +/- se) compared with 0.37 +/- 0.06 MAC in rats injected with 0.01 mg/kg of epinephrine i.p. and 0.38 +/- 0.03 MAC in rats injected with 0.1 mg/kg of epinephrine i.p. For desflurane, the amnestic ED(50) were 0.32 +/- 0.05 MAC in control rats receiving a saline injection i.p. versus 0.36 +/- 0.04 MAC in rats injected with 0.1 mg/kg of epinephrine i.p. We conclude that exogenous epinephrine does not decrease amnesia produced by inhaled isoflurane or desflurane, as assessed by fear conditioning to a tone in rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anesthesia, Inhalation
  • Anesthetics, Inhalation / pharmacology*
  • Animals
  • Desflurane
  • Epinephrine / pharmacology*
  • Fear*
  • Isoflurane / analogs & derivatives*
  • Isoflurane / pharmacokinetics
  • Isoflurane / pharmacology*
  • Learning / drug effects*
  • Male
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Anesthetics, Inhalation
  • Desflurane
  • Isoflurane
  • Epinephrine