Electron transfer dissociation of peptide anions

J Am Soc Mass Spectrom. 2005 Jun;16(6):880-2. doi: 10.1016/j.jasms.2005.01.015. Epub 2005 Apr 14.

Abstract

Ion/ion reactions of multiply deprotonated peptide anions with xenon radical cations result in electron abstraction to generate charge-reduced peptide anions containing a free-radical site. Peptide backbone cleavage then occurs by hydrogen radical abstraction from a backbone amide N to facilitate cleavage of the adjacent C-C bond, thereby producing a- and x-type product ions. Introduction of free-radical sites to multiply charged peptides allows access to new fragmentation pathways that are otherwise too costly (e.g., lowers activation energies). Further, ion/ion chemistry, namely electron transfer reactions, presents a rapid and efficient means of generating odd-electron multiply charged peptides; these reactions can be used for studying gas-phase chemistries and for peptide sequence analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Anions / chemistry
  • Electron Transport
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Peptides / chemistry*
  • Static Electricity

Substances

  • Anions
  • Peptides