A transcription-dependent DNase I-hypersensitive site in a far upstream segment of the chicken alpha-globin gene domain coincides with a matrix attachment region

Biochem Biophys Res Commun. 1992 May 15;184(3):1226-34. doi: 10.1016/s0006-291x(05)80013-0.

Abstract

A site of hypersensitivity to DNase I has been found at the 5'-side of the chicken alpha-globin gene domain, ca. 8 Kbp upstream to the first gene. The presence of this site in different types of chicken cells correlates with the transcriptional status of the domain. A matrix attachment region (MAR) has also been found in the same subfragment, suggesting that it may be involved in the control of transcription of the chicken alpha-globin genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alpharetrovirus / genetics
  • Animals
  • Binding Sites
  • Cell Line
  • Cell Line, Transformed
  • Cells, Cultured
  • Chickens
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA / metabolism
  • Deoxyribonuclease I
  • Erythrocytes / physiology
  • Genes*
  • Globins / genetics*
  • Globins / metabolism
  • Molecular Weight
  • Multigene Family*
  • Nuclear Matrix / metabolism*
  • Restriction Mapping
  • Transcription, Genetic*

Substances

  • Globins
  • DNA
  • Deoxyribonuclease I