Rotary culture enhances pre-osteoblast aggregation and mineralization

J Dent Res. 2005 Jun;84(6):542-7. doi: 10.1177/154405910508400611.

Abstract

Three-dimensional environments have been shown to enhance cell aggregation and osteoblast differentiation. Thus, we hypothesized that three-dimensional (3D) growth environments would enhance the mineralization rate of human embryonic palatal mesenchymal (HEPM) pre-osteoblasts. The objective of this study was to investigate the potential use of rotary cell culture systems (RCCS) as a means to enhance the osteogenic potential of pre-osteoblast cells. HEPM cells were cultured in a RCCS to create 3D enviroments. Tissue culture plastic (2D) cultures served as our control. 3D environments promoted three-dimensional aggregate formations. Increased calcium and phosphorus deposition was significantly enhanced three- to 18-fold (P < 0.001) in 3D cultures as compared with 2D environments. 3D cultures mineralized in 1 wk as compared with the 2D cultures, which took 4 wks, a decrease in time of nearly 75%. In conclusion, our studies demonstrated that 3D environments enhanced osteoblast cell aggregation and mineralization.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcification, Physiologic / physiology*
  • Calcium / metabolism
  • Cell Aggregation / physiology
  • Cell Culture Techniques / methods*
  • Coloring Agents
  • Electron Probe Microanalysis
  • Humans
  • Mesoderm / cytology
  • Microscopy, Electron, Scanning
  • Osteoblasts / physiology*
  • Osteogenesis / physiology
  • Palate / embryology
  • Phosphorus / metabolism
  • Rotation
  • Time Factors

Substances

  • Coloring Agents
  • Phosphorus
  • Calcium