Abstract
A simple and effective method to prepare an enzyme electronic biosensor by immobilizing enzyme nanoparticles directly onto the gold electrode surface is described; prepared horseradish peroxidase nanoparticles have been successfully used to develop reagentless electronic biosensors for H2O2 detection without promoters and mediators and offer great potential to develop enzyme-based electronic biosensors.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biosensing Techniques*
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Electrodes
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Enzymes, Immobilized / chemistry*
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Gold / chemistry
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Horseradish Peroxidase / chemistry*
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Hydrogen Peroxide / analysis*
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Microscopy, Electron, Transmission
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Nanostructures / chemistry*
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Nanostructures / ultrastructure
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Spectrophotometry, Ultraviolet
Substances
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Enzymes, Immobilized
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Gold
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Hydrogen Peroxide
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Horseradish Peroxidase