Abstract
Structural and biochemical studies have begun to elucidate the pathway of RNA silencing that leads to the formation of the RISC complex. The outstanding feature of this pathway is the precise recognition and processing of double-stranded RNA. We present a review of recent structures that illustrate the molecular mechanisms contributing to these two related functions, highlighting models of Drosha, Dicer, and RISC function.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Binding Sites
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Catalytic Domain
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Crystallography, X-Ray
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Gene Silencing
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Genetic Techniques
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Humans
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MicroRNAs / chemistry
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Models, Molecular
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Protein Conformation
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Protein Structure, Tertiary
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Pyrococcus / metabolism
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RNA / chemistry*
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RNA Interference*
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RNA, Double-Stranded / chemistry
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RNA-Induced Silencing Complex / physiology
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Ribonuclease H / chemistry
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Ribonuclease III / chemistry*
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Ribonuclease III / physiology
Substances
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MicroRNAs
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RNA, Double-Stranded
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RNA-Induced Silencing Complex
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RNA
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DROSHA protein, human
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Ribonuclease III
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Ribonuclease H