Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens

Catriona Halliday; Qi Xuan Wu; Gregory James; Tania Sorrell

doi:10.1128/JCM.43.10.5366-5368.2005

Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens

J Clin Microbiol. 2005 Oct;43(10):5366-8. doi: 10.1128/JCM.43.10.5366-5368.2005.

Authors

Catriona Halliday¹, Qi Xuan Wu, Gregory James, Tania Sorrell

Affiliation

¹ NHMRC Clinical Center of Research Excellence in Infectious Diseases, Hematology, and Health Ethics, University of Sydney Western Clinical School, New South Wales 2145, Australia. catrionah@icpmr.wsahs.nsw.gov.au

Abstract

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aspergillosis / diagnosis*
Aspergillosis / microbiology*
Aspergillus / genetics
Aspergillus / isolation & purification*
Blood / microbiology
Bronchoalveolar Lavage Fluid / microbiology
DNA Probes
DNA, Fungal / analysis*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors

Substances

DNA Probes
DNA, Fungal