Abstract
In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / metabolism
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Bromine / chemistry
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Fluorescence
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Fluorescent Antibody Technique, Indirect
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HeLa Cells
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Microscopy, Electron
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Microtomy*
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RNA, Neoplasm / metabolism
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RNA, Neoplasm / ultrastructure*
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Sensitivity and Specificity
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Tissue Embedding / methods
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Tissue Fixation
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Uridine / immunology
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Uridine / metabolism
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Water / chemistry
Substances
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Antibodies
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RNA, Neoplasm
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Water
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Bromine
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Uridine