Abstract
The yeast cell type regulator alpha 1 cooperates with a constitutive factor, MCM1 protein, to recognize the promoter and activate transcription of several alpha-specific genes. I show here that the alpha 1 and MCM1 proteins bind specifically to one of the two strands of their recognition sequence. This single-strand-binding activity shares several characteristics with the duplex-binding properties of these proteins: (i) the MCM1 protein binds alone to single-stranded and duplex sequences of both the alpha-specific (P'Q) and a-specific (P) binding sites; (ii) the alpha 1 protein requires both the MCM1 protein and the Q sequence to bind either single-stranded or duplex DNA; (iii) the alpha 1 protein stimulates binding of the MCM1 protein to both single-stranded and duplex DNAs; and (iv) the affinities of the proteins for single-stranded and duplex DNAs are comparable.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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DNA, Fungal / metabolism*
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / isolation & purification
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Fungal Proteins / metabolism*
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Gene Expression Regulation, Fungal*
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Genes, Fungal*
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Kinetics
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Mating Factor
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Minichromosome Maintenance 1 Protein
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Peptides / genetics
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Peptides / metabolism*
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Restriction Mapping
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Minichromosome Maintenance 1 Protein
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Oligodeoxyribonucleotides
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Peptides
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Transcription Factors
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Mating Factor