The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site

Mol Cell Biol. 1992 Aug;12(8):3573-82. doi: 10.1128/mcb.12.8.3573-3582.1992.

Abstract

The yeast cell type regulator alpha 1 cooperates with a constitutive factor, MCM1 protein, to recognize the promoter and activate transcription of several alpha-specific genes. I show here that the alpha 1 and MCM1 proteins bind specifically to one of the two strands of their recognition sequence. This single-strand-binding activity shares several characteristics with the duplex-binding properties of these proteins: (i) the MCM1 protein binds alone to single-stranded and duplex sequences of both the alpha-specific (P'Q) and a-specific (P) binding sites; (ii) the alpha 1 protein requires both the MCM1 protein and the Q sequence to bind either single-stranded or duplex DNA; (iii) the alpha 1 protein stimulates binding of the MCM1 protein to both single-stranded and duplex DNAs; and (iv) the affinities of the proteins for single-stranded and duplex DNAs are comparable.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • DNA, Fungal / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal*
  • Kinetics
  • Mating Factor
  • Minichromosome Maintenance 1 Protein
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Peptides / genetics
  • Peptides / metabolism*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Minichromosome Maintenance 1 Protein
  • Oligodeoxyribonucleotides
  • Peptides
  • Transcription Factors
  • Mating Factor