CART (cocaine- and amphetamine-regulated transcript) peptide receptors: specific binding in AtT20 cells

Eur J Pharmacol. 2005 Dec 28;528(1-3):188-9. doi: 10.1016/j.ejphar.2005.11.041. Epub 2005 Dec 5.

Abstract

Given previous evidence for CART (cocaine- and amphetamine-regulated transcript) signaling in AtT20 cells, the binding of [125I]-CART61-102 was characterized in these cells. The binding was specific, saturable, dependent on time, pH, temperature and protein concentration, with a Bmax of 101.4+/-8.8 fmol/mg protein and a Kd of 21.9+/-8.0 pM. Only active CART55-102, but not other peptides or drugs, inhibited the [125I]-CART61-102 binding. These data are the first demonstration of specific receptor binding for CART peptides.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Binding, Competitive
  • Cell Line / metabolism*
  • Cocaine- and Amphetamine-Regulated Transcript Protein
  • Hydrogen-Ion Concentration
  • Kinetics
  • Mice
  • Nerve Tissue Proteins / metabolism*
  • Peptide Fragments / metabolism
  • Receptors, Cell Surface / metabolism*
  • Temperature

Substances

  • Nerve Tissue Proteins
  • Peptide Fragments
  • Receptors, Cell Surface
  • Cocaine- and Amphetamine-Regulated Transcript Protein
  • cocaine- and amphetamine-regulated transcript protein (55-102)