In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

Nat Methods. 2006 Jan;3(1):27-9. doi: 10.1038/nmeth843.

Abstract

MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Mammalian / chemistry*
  • Embryo, Nonmammalian / chemistry
  • Gene Expression Profiling / methods
  • In Situ Hybridization / methods*
  • Mice
  • MicroRNAs / analysis*
  • Oligonucleotide Probes / chemistry*
  • Oligonucleotides
  • Oligonucleotides, Antisense / chemistry*
  • Zebrafish / genetics

Substances

  • MicroRNAs
  • Oligonucleotide Probes
  • Oligonucleotides
  • Oligonucleotides, Antisense
  • locked nucleic acid