Nanotechnology will play a key role in the post-genome sequencing era, since even revolutionary methods will be able to develop on a nanodevice, and it is applicable to the analysis of DNA, mRNA, protein, and other biomolecules. Recent progress in nanotechnology based on nanofabrication, nanocoating, and molecular nanotechnology is expanding the possibility of nanobiotechnology from genomics and proteomics to medical applications, including preventive medicine based on point-of-care test. Nanotechnology including quantum dot, photonic crystal, DNA chip as well as biodevice is applied to the analysis of genome network in some disease related cells. Genes related to important biological function of cell are identified by stimulating with different drugs and the genome networks for these cell reactions are predicted by computer software. Nanotechnology has been proved to be extremely important for future personalized medicine and systems biology.